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Nutrient Broth Granule

microbial medium
Chinese Name:营养肉汤(NB)颗粒
Product No.:HBKP0108
Specification:250g
Product Price:
Product Use:Used for the cultivation and the enumeration of non-fastidious microorganisms in water, food, sewage, fecal and other materials.
Remarks:

Technical Data:


Approximate Formula:

Ingredients

gm/liter

Peptic Digest of Animal Tissue

10.0

Beef Extract

3.0

Sodium Chloride

5.0

Final pH 7.2±0.2 at 25

*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend18.0gof the medium in one liter of distilled water or deionized water. Mix well and heat with frequent agitation. Sterilize in an autoclave at121℃(15 lbs.) for 15 minutes.

Principle and Interpretation:

Peptic digest of animal tissue and beef extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.

Appearance:

Dehydrated medium is a free-flowing yellowish granule.

Precautions:

This medium is for laboratory use only.

Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

Nutrient Broth must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Inoculate and incubate at 36±1℃for 18-24 hours.

Microorganism

Strains Number

Inoculum (CFU)

Growth

Remarks

Staphylococcus aureus

ATCC 25923

10-100

Luxuriant

Turbid

Listeria monocytogenes

ATCC 19114

10-100

Luxuriant

Turbid

Escherichia coli

ATCC 25922

10-100

Luxuriant

Turbid

Salmonella typhimurium

ATCC 14028

10-100

Luxuriant

Turbid

Reference:

1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

2. Marshall (ed.). 1993. Standard methods for the examination of dairy products, 16th ed. American Public Health Association, Washington, D.C.

3. U.S. Food and Drug Administration. 2001. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md.

4. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.


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