|
Urine Orientation Chromogenic Medium (UTI) |
|
|
| Chinese Name: | 尿道定位显色培养基 |
| Product No.: | HB7013 |
| Specification: | 1000ml |
| Product Price: |  |
| Product Use: | A chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections |
| Remarks: | |
|
 Technical Data:
Formula* Per Liter Purified Water
|
Ingredients
|
gm/litre
|
|
Peptic digest of animal tissue
|
15.0
|
|
Chromogenic mix
|
4.5
|
|
Agar
|
15.0
|
|
Final pH 7.0 ± 0.2 at 25℃
|
|
|
*Adjusted and/or
supplemented as required to meet performance criteria.
|
Directions
Suspend 34.5 grams of
Urine Orientation Chromogenic Medium (HB7013) in 1 litre of distilled
water. Mix well and sterilize by autoclaving at121°Cfor 15 minutes. Cool the medium to50°Cand pour into sterile Petri dishes.
Description
Urine Orientation Chromogenic Medium contains two specific chromogenic substrates which are cleaved by
enzymes produced byEnterococcus spp., E. coli and
coliforms. In addition, it contains tryptophan which indicates tryptophan
deaminase activity (TDA), indicating the presence of Proteus spp.
It is based on Cystine Lactose Electrolyte Deficient (CLED) Medium which
provides a valuable non-inhibitory diagnostic agar for plate culture of other
urinary organisms, whilst preventing the swarming of Proteus spp.
The chromogen, X-glucoside, is targeted towards ß-glucosidase enzyme activity,
and allows the specific detection of enterococci through the formation of blue
colonies.
The other chromogen, Red-Galactoside, is cleaved by the enzyme ß-galactosidase
which is produced by E. coli, resulting in pink colonies. Any
uncertainty in identification may be resolved by removing suspect colonies from
the plate and performing an indole test using Kovacs Indole Reagents (code HB8279).
The medium also contains tryptophan which acts as an indicator of tryptophan
deaminase activity (TDA), resulting in halos around the colonies of Proteus,
Morganella and Providencia spp.
Appearance:
Dehydrated Culture Medium
is a free-flowing straw -colored powder.
The prepared medium is a straw-colored, transparent agar.
Precautions:
Urine Orientation
Chromogenic Medium should only be used for in vitro diagnostic purposes. Do not
use beyond the stated expiry date, or if the product shows any sign of
deterioration.
Storage conditions and Shelf life
Urine Orientation
Chromogenic Medium must be stored tightly capped in the original container at
10-30°C. The dehydrated medium
has a shelf life of 3 years from date of manufacture. Prepared medium may be
stored, out of direct light for up to 2 weeks at 2-8°C.
Quality control
|
Test Organisms
|
Incubation
|
Results
|
|
Time
|
Temperature
|
Atmosphere
|
|
Escherichia coli
ATCC® 25922
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; medium sized rose to magenta colonies
with
darker pink centers
|
|
Klebsiella pneumoniae
ATCC® 13883
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; large, deep blue or dark indigo colonies
|
|
Enterococcus faecalis
ATCC® 29212
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; small, teal to turquoise colonies
|
|
Proteus mirabilis
ATCC® 12453
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; clear to light yellow colonies
with golden-orange
color diffused through surrounding media
|
|
Staphylococcus aureus
ATCC® 25923
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; opaque, cream to white colored
colonies
|
|
Pseudomonas aeruginosa
ATCC® 27853
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; colorless to light yellow-green,
translucent
colonies, which may have a slight
iridescence
|
|
Staphylococcus saprophyticus
ATCC® 15305
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; opaque, pink colonies
|
|
Candida albicans
ATCC® 10231
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; small, white,moist colonies
|
|
Citrobacter freundii
ATCC® 8090
|
24hr
|
36°C±1°C
|
Aerobic
|
Growth; dark blue colonies, often with a
rose halo in the
surrounding media
|
Reference
1.Collee J. G.,
Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and cCartney, Practical
Medical Microbiology,1996, 14th Edition, Churchill Livingstone.
2.Pezzlo
M., 1998, Clin. Microbiol. Rev., 1:268-280.
3.Wilkie M. E.,
Almond M. K., Marsh F. P., 1992, British Medical Journal 305:1137-1141.
4.Friedman
M. P. et al, 1991, J. Clin. Microbiol., 29:2385-2389.
5.MurrayP., Traynor P.
Hopson D., 1992, J. Clin. Microbiol., 30:1600-1601.
6.Soriano
F., Ponte C., 1992, J. Clin. Microbiol., 30:3033-3034.
7.Merlino
et al, 1995, Abstr. Austr. Microbiol. 16(4):17-3.
|
Urine Orientation Chromogenic Medium (UTI)Related products: |
| Product number | Product name | Product specification | Directions |
| HB0114-1 | VRB-MUG Agar
VRB-MUG琼脂 | 100g | Used for solid plate detection for E. coli (GB standard) | | HB0141 | Aliz-gal Agar
茜素-β-半乳糖苷琼脂(Aliz-gal琼脂) | 100g | For the rapid detect and count coliform in food, beverage and drinking water (GB / T) | | HB5213 | MUG Medium
MUG培养基 | 100g | Detection of Escherichia coli | | HB7001 | E.Coli Chromogenic Medium
大肠杆菌显色培养基 | 1000ml | For fast, accurate detection of E. coli in food, water, milk, ice cream and meat culture for 24 hours, the colonies of E. coli are Blue-green | | HB7002 | Coliforms Chromogenic Medium
大肠菌群显色培养基 | 1000ml | For fast, accurate detection of coliforms in food, water, milk, ice cream and meat, culture for 24 hours, the colonies of coliforms are blue-green | | HB7003 | E.Coli & Coliforms Chromogenic Medium
大肠杆菌／大肠菌群显色培养基(第二代) | 1000ml | Used for fast, accurate simultaneous detection of E. coli and Coliform bacteria, incubated for 24 hours, E. coli was blue-green - purple, coliform is red | | HB7004 | TBX Medium
TBX培养基 | 1000ml | For fast, accurate detection of E. coli in food, water, milk, ice cream and meat culture for 24 hours, the colonies of E. coli are Blue-green | | HB7005 | Total Genes Chromogenic Medium
细菌总数显色培养基 | 1000ml | For fast, accurate detection of total bacterial count in food, water and the environment, culture for 24 hours, bacterial colonies are red | | HB7006 | E.Coli O157:H7 Chromogenic Medium
O157显色培养基 | 1000ml | For rapid detection. E.Coli O157: H7 in food and Patient faecal samples. | | HB7007-1 | Salmonella Chromogenic Medium (GenerationⅡ)
沙门氏菌显色培养基(第二代) | 1000ml | For the rapid detection of food, water, milk, ice cream and meat and other samples of Salmonella. | | HB7008 | Listeria Chromogenic Medium
李斯特氏菌显色培养基 | 1000ml | Used for rapid detection of Listeria monocytogenes in food and medicine. Negative results can be detected completely in three days. The colonies of Listeria monocytogenes is blue, surrounded by an opaque ring. | | HB7009 | Staphylococcus aureus Chromogenic Medium
金黄色葡萄球菌显色培养基 | 1000ml | For food, rapid detection of Staphylococcus aureus in water, milk, ice cream and meat products, the results will turn out within 24 hours. The colonies of Staphylococcus aureus are blue-green | | HB7010-1 | Mold and Yeast Chromogenic Medium
霉菌和酵母菌显色培养基 | 1000ml | According to enzyme substrate method, mold and yeast chromogenic medium distinguish between different molds and yeasts by color | | HB7011 | Vibrio Chromogenic Medium
弧菌显色培养基 | 1000ml | Used for the rapid detection of Vibrio parahaemolyticus in food, seafood, and fish in patient stool samples. | | HB7012 | Enterobacter Sakazakii Chromogenic Medium
阪崎杆菌显色培养基 | 1000ml | Used for rapid detection of infant formula and other food products. The colonies of Enterobacter sakazakii are blue-green colonies | | HB7014-1 | Enterococcus Chromogenic Medium
肠球菌显色培养基 | 1000ml | Used for rapid detection of food, water and environment, Enterococcus is red to purple colonies, other bacteria is colorless or yellow or inhibited | | HB7015 | Candida Chromogenic Medium
念珠菌显色培养基 | 1000ml | Used for the isolation and identification of Candida, the colonies of Candida albicans are blue-green | | HB7018 | Shigella Chromogenic Medium
志贺氏菌显色培养基 | 1000ml | Specially used for the rapid detection of Shigella in food and patient stool samples. The colonies of Shigella are white or colorless, some sonnei Shigella have blue center | | HB7021-2 | Bacillus Cereus Chromogenic Medium (Generation Ⅲ)
蜡样芽孢杆菌显色培养基（第三代） | 1000ml | Used for the rapid detection of Bacillus cereus in food, water, dairy products and meat products. | | HB8499 | MUGal Broth
MUGal肉汤 | 100g | For rapid detection of coliforms counts in food, beverage and drinking water | | HB8558-1 | MUG Nutrient Agar
MUG营养琼脂（NA-MUG) | 100g | Used for detection Escherichia coli in drinking water and water resource by filter membrane method. | | HB8558 | MUG Nutrient Agar
MUG营养琼脂（NA-MUG) | 250g/500g | Used for detection Escherichia coli in drinking water and water resource by filter membrane method. | | HB8559-1 | EC-MUG Medium
EC-MUG培养基 | 100g | For the detection of E. coli in drinking water and water resource. | | HB8559 | EC-MUG Medium
EC-MUG培养基 | 250g/500g | For the detection of E. coli in drinking water and water resource. | | HB8569-1 | MMO-MUG Medium
MMO-MUG培养基 | 250g/500g | For synchronously test coliform and E.coli in drinking water and water resource. | | HB8569-2 | MMO-MUG Medium
MMO-MUG培养基 | 100g | For coliforms and Escherichia coli testing in drinking water and water resource | | HB8686 | MI Medium
MI培养基 | 1000ml | Used for Escherichia coli and coliform bacteria count in drinking water by membrane filtration method. | | HB8691 | MEI Medium
MEI培养基 | 1000ml | Used for chromogenic detection by one-step membrane filtration or count of enterococci in water |
| |
|
