Coliforms Chromogenic Medium-Product Details-qingdao hopebio

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    Coliforms Chromogenic Medium

    microbial medium
    Chinese Name:大肠菌群显色培养基
    Product No.: HB7002
    Specification:1000ml
    Product Price:
    Product Use:For fast, accurate detection of coliforms in food, water, milk, ice cream and meat, culture for 24 hours, the colonies of coliforms are blue-green
    Remarks:

    Technical Data:

    Coliforms Chromogenic Medium is used for fast, accurate detection of coliforms in food, water, milk, ice cream and meat, culture for 24 hours, the colonies of coliforms are blue-green.

    Ingredients (g/L):

    Special Nutrients

    34.55

    Chromogenic reagent

    0.35

    Agar

    13.0

    Final pH 7.0-7.2 at 25℃

    *Adjusted and/or supplemented as required to meet performance criteria.

    Directions:

    Suspend 47.9g of the medium in 1L of distilled water, boil in 1 minute, dispensing into flasks, at 121 ℃ autoclaving for 15 minutes, add 1mL prepared sample into a sterile in 9cm petri dish, transfer 15mL chromogenic medium when cooling down to 45-50 ℃, mix, inverted culture at 36 ± 1 ℃ for 18-24h after solidification

    Or when cooling down to 45-50 ℃, pour into a sterile petri dish, after complete solidification, inverted culture at 37 ℃ in incubator for 1-2 hours, add 1mL uniform liquid sample with a suitable concentration, streak onto the medium , culture at 36 ± 1 ℃ for 18-24h.

    Procedures:

    1. Prepared sample solution according to national standards, SN standard, FDA standards or other methods.

    2. Transfer 1mL sample onto chromogenic medium cooling down to 45-50 ℃, mix thoroughly or coating on plate;

    3. Culture at 36 ± 1 ℃ for 18-24h, select the plate which have 30 to 200 typical colonies of E. coli, total count of the colonies on the plate, typically E. coli colonies blue-green, others yellow or colorless colonies.

    4. For undermined colonies, streak them on nutrient agar plates, culture at 36 ± 1 ℃ for 12-16h, select single colony to do BGLB Fermentation tests.

    Quality Control:

    1. Appearance:

    The dehydrate powder medium is homogeneous, having good flowability, milky white, prepared medium is colorless solid.

    2. Microbial sensitivity test:Incubated at 36 ± 1 ℃ 18-24h:

    QC Strains

    Strains No.

    Growth

    Colonies color

    Listeria monocytogenes

    ATCC27853

    -/+

    colorless

    Staphylococcus aureus

    ATCC25923

    -/+

    colorless

    E. coli

    ATCC25922

    +++

    blue

    salmonella

    ATCC14028

    +++

    colorless

    Enterobacter aerogenes

    ATCC35029

    +++

    blue

    Enterobacter cloacae

    ATCC23355

    +++

    blue

    Freundii Citrobacter

    ATCC43864

    +++

    blue

    Klebsiella pneumoniae

    ATCC10031

    +++

    blue

    Methods limitations:

    The medium identification of Coliforms may occur false-positive in a few cases, but no false-negative, sometimes it needs to do other complementary tests

    Typical features:

    Coliforms typical colonies are blue-green, coliforms colonies are colorless, other bacteria are colorless or yellow colonies.

    Storage:

    The prepared plates must be used immediately, avoid direct exposure to light. The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

    Failure:

    Dry powder media which are over the shelf life, caking and color variation cannot be used.

    Note: This medium is only for laboratory use.

    Reference:

    1. FRAMPTON, E.W., RESTAINO, L. a. BLASZKO, L.: evalsuation of ß-glucuronidase substrate in a 24 hour direct plating method for Escherichia coli.  J. Food Protection, 51; 402-404 (1988).

    2. KILIAN, M. a. BÜLOW, P.: Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases. Acta Pathol. Microbiol. Scand. Sect. B 84; 245-251 (1976).

    3. LE MINOR, L. a. HAMIDA, F. BEN: Advantages de la recherche de la ßgalactosidase sur celle de la fermentation du lactose en milieu complexe dans le diagnostic bactériologique, en particulier des Enterobacteriaceae. Ann. Inst. Pasteur (Paris), 102; 267-277 (1962).


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